in silico identification of potential american cockroach (periplaneta americana) allergens

نویسندگان

a ahmed

k minhas

namood-e-sahar

o aftab

چکیده

background : cockroaches have been recognized as a powerful indoor allergen. cockroach allergy can be a major factor in seri­ous asthma and nasal allergy. bioinformatics tools have been developed to identify potential allergens. the present study was conducted to identify potential allergens in  periplaneta americana (linnaeus). methods : the study focused on the identification of potential allergens among the characterized proteins of p. americana us­ing web-based and publicly available allergen prediction tools that follow the fao/who guidelines for prediction of aller­genic proteins. p. americana protein sequences were retrieved from uniprotkb. the sequences obtained were analyzed us­ing algpred. the potential allergens obtained were further analyzed by sdap for confirmation. results : protein sequences (233 cases) of p. americana were obtained from uniprotkb out of which 25 were known aller­gens. out of the remaining 208 proteins, 102 potential allergens were predicted by algpred. however, only 9 were found to be potential allergens after screening with sdap. arginine kinases, rna polymerase ii subunit, parcxpwnx02 , p eptidyl-pro­lyl cis-trans isomerase, hemocyanin subunit type i and type ii, homologue of sarcophaga proteinase and alpha amylase  were confirmed to be potential allergens by sdap. conclusion : we have identified nine potential allergens in p. americana that may be used as preliminary support for further labo­ratory experiments.

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In Silico Identification of Potential American Cockroach (Periplaneta americana) Allergens

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عنوان ژورنال:
iranian journal of public health

جلد ۳۹، شماره ۳، صفحات ۱۰۹-۱۱۵

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